Tempo de leitura: menos de 1 minuto
TRPM3 is a nociceptor channel involved in the detection of noxious heat. Oxaliplatin administration increases expression of the voltage-dependent calcium channel 2-1 subunit in the rat spinal cord. And more precise technologies have become available as well, such as using CRISPR to targeting rat spermatogonial stem cells. doi: 10.1016/j.pain.2013.06.015, Keywords: pain, rodent, hyperalgesia, allodynia, spontaneous pain, mechanical, heat, cold, Citation: Deuis JR, Dvorakova LS and Vetter I (2017) Methods Used to Evaluate Pain Behaviors in Rodents. While not in routine clinical use, quantitative sensory testing has the potential to improve patient outcomes by classifying pain based on the mechanism and choosing treatments that target that mechanism (Baron et al., 2010; Cruz-Almeida and Fillingim, 2014). doi: 10.1002/art.21403, Miller, A. L., and Leach, M. C. (2015). The pressure is increased until withdrawal or vocalization occurs. (2014). (2011). Rock, M. L., Karas, A. Joint Bone Spine 72, 359371. doi: 10.1038/nrn2606, Moqrich, A., Hwang, S. W., Earley, T. J., Petrus, M. J., Murray, A. N., Spencer, K. S., et al. Under the direction of the researcher, the apparatus applies a von Frey (0.5 mm) filament to the plantar surface, increasing the force incrementally (050 g) until the paw withdrawal threshold is reached. Given the variability in cold pain thresholds, it may be difficult to differentiate between cold allodynia and cold hyperalgesia in the clinic. 3. PDF A Guide to Research Rodent Housing - Charles River Laboratories A disadvantage of this method is that the number of measurements per animal is variable and that it requires repeated, time-intensive measurements, which may lead to sensitization or learnt responses. doi: 10.1093/neuonc/nou048, Deuis, J. R., and Vetter, I. (2009). Pain 68, 133140. Protoc. In recent years mice have been used with increasing frequency, to take advantage of genetically modified lines as well as the relatively lower costs compared with rats.
Majestic Princess Premium Deluxe Balcony,
What Happened With Paul Keith And Wanda Davis,
Articles OTHER
13840110d2d515487e83b87 disadvantages of using rats in research